Baseline: 8+ weeks fully off everything (including OTC test boosters).
Mid-cycle: Week 6–8 of a blast. Draw at trough — right before your next pin, fasted, AM.
Post-cycle: 4 weeks after last pin (long esters), or 2 weeks (short esters / orals).
Cruise check-in: Every 12 weeks while on TRT or cruise.
No training 48h before draw. No sauna, no alcohol, no creatine loading. Fasted 10–12h, water only.

THE FULL PANEL

•Total Testosterone (LC/MS)

•Free Testosterone (equilibrium dialysis)

•Estradiol (sensitive, LC/MS)

•SHBG

•Prolactin

•LH

•FSH

•DHT

•Progesterone

•CBC w/ differential

•Hematocrit

•Hemoglobin

•RBC

•CMP (liver, kidney, electrolytes)

•ALT

•AST

•GGT

•Bilirubin

•Lipid panel

•ApoB

•Lp(a) (once)

•hs-CRP

•Homocysteine

•HbA1c

•Fasting glucose

•Fasting insulin

•TSH

•Free T3

•Free T4

•Cystatin C

•eGFR

•Urinalysis

•IGF-1 (if on GH/peptides)

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SIDE-EFFECT TROUBLESHOOTING

The stuff you desperately search for and never find explained right.

LIKELY CAUSE

Low estrogen from heavy AI use + harsh androgens (Tren, Mast, Winstrol) drying the joints. Low E2 = no lubrication, no collagen synthesis support.

FIX

→Pull E2 with sensitive assay — if under 20 pg/mL, back off the AI.
→Drop Winstrol first. It's the worst joint offender at any meaningful dose.
→Add 5g collagen + 50mg vit C pre-training. Doesn't fix the hormonal cause but buys time.
→If joints crunch even with E2 in range, you're under-eating fat. Cutting ≠ zero fat.

SLEEP, STRESS & CORTISOL

The silent killer of results nobody coaches you on.

SLEEP PROTOCOL

→ Hard cutoff on caffeine 10 hours before bed. Half-life is 5–6h.
→ Last meal 3 hours before bed. Carb-heavy, low fat.
→ Bedroom: 65–68°F, blackout, white noise.
→ Magnesium glycinate 400mg + glycine 3g + apigenin 50mg, 45 min before bed.
→ Same bedtime ±30 min, 7 days a week. Weekend drift kills you.
→ No screens last 30 min, or wear blue-blockers from sundown.
→ Wake same time, sunlight in eyes within 10 min.

STRESS PROTOCOL

→ 10 min daily: nasal breathing, 4-7-8 cadence. Non-negotiable.
→ 1 zero-screen meal per day. Eat slow, no phone.
→ Weekly Z2 cardio 3–4x, 30+ min. Lowers baseline cortisol.
→ Sauna 4x/week, 20 min @ 175°F+. Heat shock protein + parasympathetic.
→ Cut alcohol to 0 during a blast. It tanks sleep, jacks cortisol.
→ Audit your inputs: news, social, group chats. Mute the noise.
→ Sunday 30 min: plan the week. Decision fatigue = cortisol.

CORTISOL PROTOCOL

→ Test AM cortisol (7–9 AM) + 4-point salivary if symptoms persist.
→ AM high / PM low = ideal. Flat or reversed = problem.
→ Bright light AM, dim light PM. Anchors the rhythm.
→ Carbs at night (50–100g) blunt nighttime cortisol spikes.
→ Ashwagandha 600mg/day (KSM-66) for 60 days, then reassess.
→ Phosphatidylserine 300mg PM if cortisol stays elevated at night.
→ Cutting too hard? Calories under 1.2g/lb? You're the problem. Eat.

Educational only. None of this is medical advice. Work with a doctor who actually knows what you're doing.

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